Publication date:

2018

Abstract

In Iran, during significant research, the importance of the antagonistic fungus,Talaromyces flavus has been known for the inhibition of the growth of some important plant soil pathogens such as Rhizoctonia solani, Verticillium dahliae and Fusarium oxysporum. Also, according to the results of previous studies, preparation of the above-mentioned fungal bioformulations with substrates in the form of different plantresidues such as rice bran, wheat straw and corn wood was possible and using them in the greenhouse and field, in addition to reducing the incidence of the diseases such as the seedling death, Verticillium and Fusarium wilt in cotton, sugar beet, potato, tomato and greenhouse cucumber, increased yield was also observed. According to the results obtained to this stage of the research, the commercialization of the bioformulation of this fungus is of particular importance. Since the marketing is considered as one of the important factors for the continuation of commercialization, considering the type of bioformulation with easy applications can greatly affect the attraction of relevant consumers and a successful marketing. Therefore, in light of the recent advances in the application of nanotechnology in various sciences, it seems necessary to prepare different nanoformulations of the above biological agent with an emphasis on the ease of use, and study theefficacy in biological control of the plant diseases. In this study, nanoformulations were prepared in two types: nanocapsule (F1 and F3), a type of nanoemulsion (F2), and a type of powder nanoformulation (F4) of T. flavus.

Author: Laleh Naraghi, Maryam Negahban

Keywords: Nanoformulation, Nanopesticide, Nanotechnology, Penicillium dangeardii, Talaromyces flavus 

Publication date:

2018

Abstract

The importance of the Talaromyces flavus antagonistic fungus has been understood for inhibiting the growth of some of the important plant pathogenic agents such as Rhizoctonia solani, Verticillium dahliae and Fusarium oxysporum. Additionally, based on the results of previous research, preparation of the mentioned fungal bioformulation was possible with substrates in the form of various plant residues such as rice bran and wheat straw. The application of this bioformulation in the greenhouse and the field, in addition to reducing the incidence of seedling damping-off, Verticillium wilt and Fusarium wilt diseases in cotton, sugar beet, potato, tomato and greenhouse cucumber, while increases their yields. According to the obtained results, the commercialization of the bioformulation of this fungus has particular importance. Since the marketing is considered to be an important factor in the continuation of commercialization, considering the type of bioformulation with ease of use can greatly affect the attraction of relevant consumers and a successful marketing. Therefore, due to the recent advances in the application of nanotechnology in various sciences, it seems necessary in the further research to investigate different nanoformulations from the biological agent with an emphasis on the ease of use, preparation and their efficacy in biological control of plant diseases. In this study, nanoformulations were prepared in three types: nanocapsule (F1 and F3), nanoemulsion (F2), and nanopowder (F4). Three and six months after production, laboratory experiments on nanoformulations and the formulation based on previous technical knowledge (rice bran and T. flavus) on sporulation and active population of the antagonistic fungus were carried out in a completely randomized design. The results showed that there was a significant increase in sporulation and active population in nanopowder compared to other formulations.

Author: Laleh Naraghi, Maryam Negahban, Asghar Heydari, Mohammad Razavi, Homayoun Afshari-Azad

Keywords: Active Population, Nanoparticle, Talaromyces Flavus, Sporulation

 

Publication date:

2018

Abstract

In Iran, during conducting significant research, the importance of Talaromyces flavus antagonistic fungus has been known to inhibit the growth of some of the important soilborne plant pathogens such as Rhizoctonia solani, Verticillium dahliae and Fusarium oxysporum. Also, according to the results of previous studies, preparation abovementioned fungal bio formulation was possible with substrates in the form of various plant residues such as rice bran, wheat straw and corn wood using them in the greenhouse and field, in addition to reducing. The occurrence of diseases such as the seedling death, Verticillium and Fusarium wilt in cotton, sugar beet, potato, and greenhouse tomato and cucumber, increased yield also observed. According to the results obtained to this stage of the research, the commercialization of the bio-formulation of this fungus is very importance. Since the marketing is considered as one of the important factors for the continuation of commercialization, consideration of the type of bio formulation with easy applications can greatly affect the attraction of relevant consumers and a successful marketing. Therefore, in light of the recent advances in the application of nanotechnology in various sciences, it seems necessary in the further research to prepare different nano formulations of the biological agent with an emphasis on the ease of use, and study their efficacy
in biological control of the plant diseases. In this study, we prepared nano-bio-formulations including two types of nanocapsules (F1 and F3), a type of nano emulsion (F2), and a powder type nano formulation (F4) form T. flavus fungus. Three and six months after the production, laboratory tests of nanoformulations were performed in a completely randomized design in terms of the efficiency in inhibiting the colony growth of Verticillium dahliae. The results showed that nanopowder was more effective than other nanoformulations in term of inhibiting the colony growth of the pathogen.

Author: Laleh Naraghi, Maryam Negahban

Keywords: Inhibitory Effect, Nanoparticle, Talaromyces Flavus, Verticillium Dahliae

 

Publication date:

2018

Abstract

In recent years, different formulations such as nanoemulsions have been widely used for the target delivery, and enhanced biological functions of pesticides combinations. In this study, contact toxicity of Mentha longifolia L. essential oil compared with its nanoemulsion on Ephestia kuehniella Zeller has been investigated. The experiments were conducted and executed at 27±1 C, and 75±5% relative humidity under dark conditions. Chemical composition of this plant extract was also studied. The main constituents were pulegone (28.84%), 1, 8-cineol (19.6%), p-menthan-3-one-cis (8.2%), β-pinene (6.46%) and p-menthan-3-one-trans (5.86%). Results indicated that the mortality rate of 5th instar larva of E. kuehniella increased with rising concentrations (10000-40000ppm) for oil and (8000-20000 ppm) for nanoemulsion, respectively. The essential oil LC50 was 21352ppm, while this value for nanoemulsion was 14068ppm. According to the results, nanoemulsion was more effective than essential oil. M. longifolia oil had lower durability and the 50% persistent time (PT50) was 2.39 day compared to the nanoemulsion (PT50= 17.13 day) in the highest concentration of essential oil. The nanoparticle size was 14-36 nanometers (nm) when the transmission electron microscope (TEM) was applied.  The surface morphology of nanoemulsion was also studied by TEM. The average size of the particles was estimated 234nm by using laser light scattering apparatus. The overall results of this study show that by using nanoemulsion formulation, the effect of essential oil contact toxicity and its durability increases. Hence, the nanoemulsion slow-release formulation may represent a new category of biopesticides and this should be considered in the integrated pest management program.

Author: Mojdeh Louni, Jahanshir Shakarami, Maryam Negahban

Keywords: Contact toxicity; Ephestia kuehniella; essential oil; Mentha longifolia; Nanoemulsion

 

Publication date:

2018

Abstract

In this study, the nanoemulsion containing Mentha longifolia essential oil was produced by using high-pressure homogenization method and its fumigant toxicity was compared with ordinary M. longifolia essential oil on different stages of Ephestia kuehniella. Experimental conditions were as follows; 27±1 °C, 70± 5% relative humidity in total darkness. Concentrations of 111.1-1111.1 and 62.9- 92.6 μl/l air of oil have been used to control larva and eggs, respectively. Nanoemulsion concentrations were studied at 122.2-1111.1 μl/l air for larvicide experiment and 81.4-114.8 μl/l air for ovicidal stage. PT50 value at 1111.1 μl/l air as a larvicide for nanoemulsion and oil was estimated about 15.18 and 3.69 days, respectively. This value, at 92.6 μl/l air on egg was 14.44 and 2.58 days, respectively. The relative median potency parameter (RMP) showed that there was significant difference between PT50 values of essential oil and its nanoemulsion on both of the two stages of pest. Results showed that nanoemulsion containing M. longifolia oil can release slow properties lead to durability of fumigant toxicity of oil in the long term. Therefore, this new formulation could be considered as a new and ecofriendly biopesticide in pest control.

Author: Mojdeh Louni, Maryam Negahban, Jahanshir Shakarami

Keywords: Ephestia kuehniella, Essential oil, Durability, Mentha longifolia, Nanoemulsion

 

Publication date:

2017

Abstract

In this study, the insecticidal activity Artemisia Haussknechtii essential oil, extract and encapsulated form of essential oils in nanoparticles were investigated. Interfacial compression polymerization method was investigated for nano capsules preparation. The effects of emulsifier composition, co-emulsifier, and temperature on the properties of the nanocapsules were investigated. The scanning electron microscopy of particles proved the preparation of nanoparticles. Fumigant toxicity of oil and extract demonstrated fumigant toxicity against Tribolium castaneum and Sitophilus oryza.
It was demonstrated a mortality of 100 percent of Sitophilus oryzae in the concentration of 166ppm. The insecticidal activity varied based on insect species. With the alteration of concentrations of the oil and extracts and exposure time, the variety on fumigant toxicity had shown due to the volatility of extract. The results demonstrated that A. haussknechtii essential oil and its nano-encapsulated form could play a significant role in the formulation of essential oil-based insecticides for the management of stored-grain insects.

Author: Masumeh Khanahmadi, Parvaneh Pakravan, Abbas Hemati Azandaryani, Maryam Negahban, Elmira Ghamari

Keywords: Artemisia Haussknechtii, Essential oil, Extract, Fumigant toxicity, Contact toxicity

 

Publication date:

2017

Author: Reza Seifi, Saeid Moharramipour, Maryam Negahban, Maryam Masoumi

Keywords: Acaricidal, nano formulation, nano emulsion, nano pesticide, plant compounds